Journal: International Journal of Molecular Sciences

Article Title: BMP9 Promotes the Proliferation and Migration of Bladder Cancer Cells through Up-Regulating lncRNA UCA1

doi: 10.3390/ijms19041116

Figure Lengend Snippet: The validation of recombinant adenovirus BMP9 and siBMP9. ( A ) The expression of BMP9 in normal bladder mucosa ( n = 126), superficial bladder cancer ( n = 68), and infiltrating bladder cancer ( n = 62) in the Lee Bladder database. p = 0.007; ( B ) The expression levels of BMP9 in T24 and BIU-87 cells were detected by western blot; ( C ) The BMP9 was up-regulated in BIU-87 cells after being transfected with AdBMP9 compared to the control group; ( D ) The BMP9 was down-regulated in T24 cells after being transfected with AdsiBMP9 compared to the control group. Data are shown as mean ± SD. ** p < 0.01.

Article Snippet: Human bladder cancer BIU-87 and T24 cells were obtained from the China Center for Type Culture Collection (CCTCC).

Techniques: Biomarker Discovery, Recombinant, Expressing, Western Blot, Transfection, Control

Journal: International Journal of Molecular Sciences

Article Title: BMP9 Promotes the Proliferation and Migration of Bladder Cancer Cells through Up-Regulating lncRNA UCA1

doi: 10.3390/ijms19041116

Figure Lengend Snippet: BMP9 up-regulated the expression of lncRNA UCA1 in bladder cancer cells. ( A ) Five common lncRNA were screened in BIU-87 cells after transfected with AdBMP9 by RT-PCR; ( B ) The expression of lncRNA UCA1 were verified in BIU-87 cells after transfected with AdBMP9 by RT-PCR; ( C ) The expression of lncRNA UCA1 were tested in T24 cells after being transfected with AdsiBMP9 by RT-PCR; ( D ) The inhibitory effect of siUCA1 were analyzed by RT-PCR in BIU-87 cells after being co-transfected with AdBMP9 and siUCA1. Data are shown as mean ± SD. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, vs. control groups.

Article Snippet: Human bladder cancer BIU-87 and T24 cells were obtained from the China Center for Type Culture Collection (CCTCC).

Techniques: Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction, Control

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Cytotoxicity and Apoptosis Studies of Brucein D against T24 Bladder Cancer Cells

doi: 10.31557/APJCP.2024.25.3.921

Figure Lengend Snippet: Cytotoxic effect of BrD in T24 Cells Treated for 72 h White Arrow Indicates Morphological Changes in Treated Cells. BrD = Brucein D, Dox = Doxorubicin, Doc = Docetaxel

Article Snippet: Cell Line T24 Bladder Cancer The bladder cancer cell line T24 was purchased from the ECΑCC (European Collection of Authenticated Cell Cultures) with cat-pack number 85061107-1 VL.

Techniques:

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Cytotoxicity and Apoptosis Studies of Brucein D against T24 Bladder Cancer Cells

doi: 10.31557/APJCP.2024.25.3.921

Figure Lengend Snippet: Dose-Response of BrD Treatment (0.01 – 100 µg/mL) in T24 cells for 72 h

Article Snippet: Cell Line T24 Bladder Cancer The bladder cancer cell line T24 was purchased from the ECΑCC (European Collection of Authenticated Cell Cultures) with cat-pack number 85061107-1 VL.

Techniques:

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Cytotoxicity and Apoptosis Studies of Brucein D against T24 Bladder Cancer Cells

doi: 10.31557/APJCP.2024.25.3.921

Figure Lengend Snippet: Viability assay by Staining with (A) Calceіn AM (green) and PI (red) in T24 Cells Treated with a Graded Dose of BrD, Doxorubicin, and Docetaxel for 72 h. The percentage of cells stained with PI represents dead cells (B) The percentage of dead cells showed an increase in a dose-dependent manner (ns indicates non-significant, * significant difference p< 0.05, ** p<0.01, *** p<0.001, **** p<0.0001)

Article Snippet: Cell Line T24 Bladder Cancer The bladder cancer cell line T24 was purchased from the ECΑCC (European Collection of Authenticated Cell Cultures) with cat-pack number 85061107-1 VL.

Techniques: Viability Assay, Staining

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Cytotoxicity and Apoptosis Studies of Brucein D against T24 Bladder Cancer Cells

doi: 10.31557/APJCP.2024.25.3.921

Figure Lengend Snippet: Apoptosis-Inducing Effects of T-24 Cells: (A) Hoeϲhѕt33342 staining shows condensation of chromatin in apoptotic cells; arrowheads = apoptotic nuclei. (B) CTCF values of T-24 cells treated with BrD, doxorubicin, and docetaxel at IC50 concentrations, shows an increase in cell fluorescence that indicates an increase of chromatin condensation in all treatment group compared to control (C) Measurement of the apoptotic nuclei percentage in T24 cells treated with BrD, doxorubicin, and docetaxel in IC50 concentrations shows an increase of apoptotic nuclei percentage in all treatment group compared to control. (D) Fragmentation of T24 genomic DNA treated with BrD, doxorubicin, and docetaxel at IC50 concentration; a 1 kb DNA ladder (250–10,000 bp Τhermo Fіsher) was used as a basic measurement; M: 1 kb marker DNA ladder; C: control. Arrows indicate genomic DNA and fragmented DNA. (ns indicates non-significant, * significant difference p< 0.05, ** p<0.01, *** p<0.001, **** p<0.0001)

Article Snippet: Cell Line T24 Bladder Cancer The bladder cancer cell line T24 was purchased from the ECΑCC (European Collection of Authenticated Cell Cultures) with cat-pack number 85061107-1 VL.

Techniques: Staining, Fluorescence, Concentration Assay, Marker